Qds aroused significant interest in the bioimaging field through integrating the fluorescence properties of qds and the delivery function of biomaterial. Viruses and nanotechnology marianne manchester springer. Finally, the novel tumor targeting properties of certain viruses are harnessed to achieve specificity in vivo. September quantum dots encapsulated with canine parvovirus like particles improving the cellular targeted labeling dan yan 0 1 bin wang 0 1 shiqi sun 0 1 xia feng 0 1 ye jin 0 1 xueping yao 0 1 suizhong cao 0 1 huichen guo 0 1 0 1 state key laboratory of veterinary etiological biology and national foot and mouth disease reference laboratory, lanzhou veterinary research institute, chinese. Canine and feline parvoviruses can use human or feline transferrin receptors to bind, enter, and infect cells. Canine parvoviruslike particles, a novel nanomaterial. Recently, viruses have been explored as nano containers for specific targeting applications. In general, qds are nanoparticles with diameters of 1100 nm that are. The capsid protein vp1 is primarily responsible for the infectivity of. Mechanisms of cell death in canine parvovirusinfected. Singh 101 demonstrated that canine parvovirus nps cpvnps can be used for targeting tumor cells. Dec 01, 2006 targeted therapy using virusbased nanoparticles vnps targeted therapy using virusbased nanoparticles vnps manchester, marianne 20061201 00. Cpv, a viral pathogen of canids dogs belongs to the family parvoviridae 26.
Abstract abstract specific targeting of tumor cells is an important goal for the design of nanotherapeutics for the treatment of cancer. For example, the canine parvovirus cpv utilizes transferrin receptors. Canine parvovirus ns1 protein exhibits antitumor activity. The use of viruslike protein cages to generate novel materials that can be used for biomedical applications such as biomedical imaging and vaccine purposes is discussed. The development of specialized nanoparticles for use in the detection and treatment of cancer is increasing. Parvovirus ns1 is one of such proteins, which is known to kill high proliferating tumor cells. Multifunctional nanoparticles and cancer drug delivery. Canine parvovirus like particles, a novel nanomaterial for tumor targeting. Biomimetic strategies for targeted nanoparticle delivery dehaini. Chemical conjugation of heterologous proteins on the. Therapeutic nanoparticles for drug delivery in cancer. Acute effect of infection by adipogenic human adenovirus ad36.
Both feline parvovirus and canine parvovirus bind to and infect the transferrin receptors, but both have different sequences in the cells and animals. Nanoparticles may have properties of selfassembly, stability, specificity, drug encapsulation and biocompatibility as a result of their material composition. Structure determination of feline panleukopenia virus. The viral particles are nanostructures with nanocontainer for cellular delivery as they have naturally evolved mechanisms for binding to an entering to cells. We sought to encapsulate qds within targeted pegylated liposomes to reduce their propensity for liver. Qd photoemissions are bright, of narrow wavelength range, and very stable. To improve the biodistribution of cancer drugs, nanoparticles have been designed for optimal size and surface. Canine parvovirus and benefits of plasma treatment. Specific targeting of tumor cells is an important goal for the design of nanotherapeutics for the treatment of cancer. The advantages of using nanoparticles for drug delivery include enhanced water solubility, tumor speci. Smallmolecule delivery by nanoparticles for anticancer. Cancer nanotherapeutics are rapidly progressing and are being implemented to solve several limitations of conventional drug delivery systems such as nonspecific biodistribution and targeting, lack of water solubility, poor oral bioavailability, and low therapeutic indices. The natural tropism of canine parvovirus cpv to the transferrin receptor can target specific cells to increase the.
However, synthetic peptides when used as a vaccine, without a delivery system have been shown to be ineffective due to its rapid degradation by proteases, along with its poor cellular uptake and im. Mar 21, 2016 magnetic resonance imaging revealed splenic targeting of canine parvovirus capsid protein vp2. Many methods exist for creating targeted nanoformulations, including the use of custom. Tumor targeting using canine parvovirus cpv another recentlycharacterized vnp used for tumor targeting with a potential for intravital imaging is the canine parvovirus cpv fig. The advantages of using nanoparticles for drug delivery include enhanced water solubility, tumor specific accumulation and improved antitumor efficacy, while reducing nonspecific toxicity. Dujs 11w by dartmouth undergraduate journal of science issuu. Recently, viruses have been explored as nano containers for specific targeting applications, however these systems typically require modification of the virus surface using chemical or genetic means to achieve tumorspecific delivery. Molecular epidemiology of canine parvovirus, europe nicola decaro, costantina desario, diane d. Tumor targeting using canine parvovirus nanoparticles a biodegradable phsensitive micelle system for targeting acidic solid tumors november 2007 pharmaceutical research, vol. Expansion of tropism of a feline parvovirus to target a human. Investigation of the antiviral effect of acyclovir on canine. Receptortargeted cancer therapy dna and cell biology.
Towards this goal, cpv viruslike particles vlps produced by expression of the cpvvp2 capsid protein in a baculovirus expression system were examined for attachment of small molecules and delivery to tumor cells. Parvoviruses have been used as an antitumor agent, immune cell activator, and as a targeted gene vector. However, its broad host range may limit its usefulness and effectivity in several gene therapy applications in which transgene expression needs to be limited to a specific organ or cell type. Since tfr recognition relies on the cpv capsid protein, we envisioned the use of virus or its shells as tumor targeting agents. Canine parvovirus cpv, cpv2 and feline panleukopenia virus fpv are very closely related and are important pathogens of their respective hosts, the dog and cat. Canine parvoviruslike particles, a novel nanomaterial for tumor targeting. Magnetic resonance imaging revealed splenic targeting of canine parvovirus capsid protein vp2. Incorporation of tumortargeting peptides into recombinant. We observed that derivatization of cpv viruslike particles vlps with dye molecules did not impair particle binding to tfrs or internalization into human tumor cells. Predominance of canine parvovirus cpv in unvaccinated cat populations and emergence of new antigenic types of cpvs in cats. Caspase activation is required for permissive replication of aleutian mink disease parvovirus in vitro. One of the bestcharacterized ligands to be exploited for targeting tumor. Cpv, a viral pathogen of canids dogs belongs to the family parvoviridae.
Schematic showing the preparation of fegqd nanoparticles coated with canine parvovirus vp2 protein for mr imaging in vitro and in vivo. Targeted therapy using virusbased nanoparticles vnps. Surface mutation lets canine parvovirus jump to other species. The parvoviruses are widely spread in many species and are among the smallest dna animal viruses.
Quantum dots encapsulated with canine parvovirus like particles improving the cellular targeted labeling. Interestingly, canine parvovirus cpv has a natural affinity for transferrin receptors tfrs both of canine and human origin and this property could be harnessed as tfrs are overexpressed by a variety of human tumor cells. Preparation, characterization and immunological evaluation. Current research in this field focuses on understanding precisely how small molecules are. Dec 28, 2017 8 ways to treat and prevent parvovirus in dogs 1 vaccinations a vaccine is a substance that triggers the production of antibodies in the dogs body, which can provide immunity against disease. The infectious agent is an icosahedral t 1, nonenveloped virus encapsidating a single stranded dna of about 5 kb and shows an average diameter of 26. Role of capsid proteins in parvoviruses infection virology. The samples were subsequently tested for cpv using polymerase chain reaction pcr. In the present study, we assessed the apoptosis inducing ability of canine parvovirus type 2. This volume describes the unprecedented opportunities to capitalize on the vast knowledge of virus particles and their physical and material properties. In this study we characterized canine parvovirus cpvvlps as a potential nanomaterial for tumor targeting purposes.
Biomimetic strategies for targeted nanoparticle delivery. Targeted therapy using virusbased nanoparticles vnps manchester, marianne 20061201 00. Molecular characterization of canine parvovirus cpv. May 14, 2007 these days drug delivery using micro nano particles has been shown to have great potentials for achieving controlled and targeted therapeutic effects. We explored whether the natural tropism of cpv to tfrs could be harnessed for targeting tumor cells. Sep 23, 2015 the natural tropism of canine parvovirus cpv to the transferrin receptor can target specific cells to increase the targeting ability of qds in cell imaging. A subset of viruses with natural affinity for receptors on tumor cells could be. F maxwell and ja corsini, expansion of tropism of a feline parvovirus to target a human tumor cell line by display of an. Novel nanoparticles for specific tumor targeting nanowerk. Article pdf available in journal of nanobiotechnology 41.
Pdf quantum dots encapsulated with canine parvovirus. To overcome natural cpmv targeting and redirect cpmv particles to cells of interest, we attached a folic acidpeg conjugate by using the coppercatalyzed azidealkyne cycloaddition reaction. Isolation and molecular characterization of canine and feline. A member of the family parvoviridae that includes the genedelivery vehicle adenoassociated virus aav, cpv is a natural pathogen of dogs 110. The infectious agent is an icosahedral t 1, nonenveloped virus encapsidating a.
Icosahedral chimeric particles of this type often display novel properties resulting in high local. The natural tropism of canine parvovirus cpv to the transferrin receptor can target specific cells to increase the targeting ability of qds in cell imaging. The tumor targeting sequence ngraha and a myc epitope control were incorporated either as insertions or as replacements of the original capsid sequence. Cpv2 infects dogs and other canidae such as wolves, coyotes, south american dogs and asiatic raccoon dogs, but not cats.
The parvovirus is composed of a single strand molecule of dna wrapped into an icosahedral capsid. Investigation of the antiviral effect of acyclovir on. Canine parvovirus is a virus recognized in the 1970s that has consistently transmutated into new virulent forms. Selfassembly of viruslike particles of canine parvovirus capsid protein expressed from escherichia coli and application as viruslike particle vaccine. Cpmv interacts with several mammalian cell lines and tissues in vivo. In this study, we explored the possibility of directing. We demonstrate the use of gold nanorods as bright contrast agents for twophoton luminescence tpl imaging of cancer cells in a threedimensional tissue phantom down to 75.
Quantum dots encapsulated with canine parvoviruslike. Recombinant adenovirus type 5 vectors that target dcsign, chemr23 and. Sixtyfive samples from dogs with and without clinical signs of parvovirus infection were collected between april 2009 and february 2010. Footandmouth disease viruslike particles as integrin. Nanoscale devices have impacted cancer biology at three levels. Using nanoparticles for the delivery of small molecules in anticancer therapy is a rapidly growing area of research. The carrier particles have specific transportation and extravasation 14 behaviors determined by their chemical structure, size, and surface properties etc. Modification of the subunit genes of a variety of viruses has permitted the display of polypeptides on both the infectious virions and virus particles made in expression systems. Current topics in microbiology and immunology, vol 327. Molecular epidemiology of canine parvovirus, europe.
Pathogenic potential of canine parvovirus types 2a and 2c in domestic cats. The article can be downloaded for free here pdf download 4. Besides this artificial targeting, a subset of viruses, such as canine parvovirus, have natural affinity for receptors such as transferrin receptors that are upregulated on a variety of tumour cells 49. Canine parvovirus and benefits of plasma treatment iowa. Read canine parvovirus ns1 protein exhibits anti tumor activity in a mouse mammary tumor model, virus research on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Journal of nanobiotechnology research open access canine parvovirus like particles, a novel nanomaterial for tumor targeting pratik singh1,2, giuseppe destito1,2,4, anette schneemann1,3 and marianne manchester1,2. Magnetic resonance imaging revealed splenic targeting of. Utilizing the folate receptor for active targeting of cancer nanotherapeutics grant l. Pdf canine parvoviruslike particles, a novel nanomaterial. Request pdf magnetic resonance imaging revealed splenic targeting of canine parvovirus capsid protein vp2 canine parvovirus cpv is a highly contagious infectious virus, whose infectious. Quantum dots qds are attracting intense interest as fluorescence labeling agents for biomedical imaging because biocompatible coatings and relatively nontoxic rare earth metal qds have emerged as possible options.
The canine parvovirus has the tradeoff of gaining the ability to infect canine cells, while becoming less effective at infecting feline cells. Viruses were assessed for packaging, accessibility of incorporated peptides, heparin binding, and transduction in a range of cell lines. Apr 17, 2001 we are modifying the capsid of feline panleukopenia virus fpv, a parvovirus which normally infects feline cells, with the goal of targeting human tumor cells for potential cancer therapy. Twophoton luminescence imaging of cancer cells using. Signs of cpv infection include severe dehydration, disseminated intravascular coagulation dic, and bacterial translocation with sepsis. Apr 14, 2016 surface mutation lets canine parvovirus jump to other species. Parvoviruses have small nonenveloped virions virus particles, and the icosahedral capsid the protein shell surrounding the viral nucleic acids is made up of 32 capsomeres capsid subunits measuring 1826 nm 1 nm 10.
Addie, vito martella, maria joao vieira, gabriella elia, angelique zicola, christopher davis, gertrude thompson, ethienne thiry, uwe truyen, and canio buonavoglia canine parvovirus cpv, which causes hemorrhagic. Canine parvovirus vp2 protein expressed in silkworm pupae selfassembles into viruslike particles with high immunogenicity. By targeting heat shock protein, a dualfunction protein cage with specific targeting and doxorubicin encapsulation has been developed 50. These targeting strategies are typically not as efficient when compared to the natural cell receptor targeting potential of a virus. Three variants, namely cpv2a, 2b and 2c completely replaced the. Nanotechnology has the potential to offer solutions to these current obstacles in cancer therapies, because of its unique size and large surfacetovolume ratios. Besides this artificial targeting, a subset of viruses, such as canine parvovirus, have.
Other nanodevices have also been used in cancer therapy such as gold nps. Cpv viruslike particles vlps from the expression of the cpvvp2 capsid protein in a prokaryotic expression system were examined to encapsulate the qds and deliver to cells with an. For example, canine parvovirus vlps containing transferrin were used in a targeted delivery system based on the recognition of transferrin receptors that are overexpressed in tumor cells. For example, the canine parvovirus cpv utilizes transferrin receptors tfrs, which are overexpressed by a variety of tumour cells, for binding and entry into canine and human cancer cells singh et al. Folic acidmediated targeting of cowpea mosaic virus. These studies demonstrate that cpmv has significant potential for use in targeting and imaging of both normal vasculature and tumors in vivo but also underscores the need for cellspecific retargeting of the capsid.
Since tfr recognition relies on the cpv capsid protein. Nanoparticles as carriers for drug delivery in cancer. Recently, viruses have been explored as nanocontainers for specific targeting applications, however these systems typically require modification of the virus surface using chemical or genetic means to achieve tumorspecific delivery. Besides this artificial targeting, a subset of viruses, such as canine parvovirus, have natural affinity for. Nanoparticles have been designed for optimal size and surface. The tpl intensity from goldnanorodlabeled cancer cells is 3 orders of magnitude brighter than the twophoton autofluorescence tpaf emission intensity from unlabeled cancer cells at 760 nm excitation light. Pdf canine parvoviruslike particles, a novel nanomaterial for. Scientists are engineering viral nanoparticles, such as the cowpea mosaic virus and the canine parvovirus, to help cure cancer. The human parvovirus adenoassociated virus type 2 aav2 possesses many features that make it an attractive vector for gene delivery in vivo. After adding the product to the cart by clicking the button below you will see if there is a volume discount available. Cpv viruslike particles vlps from the expression of the cpvvp2 capsid protein in a prokaryotic expression system were examined to encapsulate the qds and deliver to cells with an expressed transferrin receptor. Genetic economy leads to symmetric distributions of chemically identical subunits in icosaherdal and helical viruses. Quantum dots encapsulated with canine parvoviruslike particles.
Tumor targeting using canine parvovirus nanoparticles. Canine parvovirus 2 cpv2 has been considered to be an important pathogen of domestic and wild canids and has spread worldwide since its emergence in 1978. Canine parvoviruslike particles, a novel nanomaterial for tumor. The samples were subsequently tested for cpv using. Canine parvoviruslike particles, a novel nanomaterial for. One of the most devastating canine diseases is canine parvovirus enteritis which is a highly contagious disease that occurs worldwide, but is still evolving. Similarly, there is a tremendous need to identify, image, and monitor tumors particularly at. This study provides data about canine parvovirus cpv types circulating among dogs in turkey. Canine parvovirus ns1 protein exhibits anti tumor activity in a mouse mammary tumor model. Parvovirus, any virus belonging to the family parvoviridae.